Department of Molecular Cell Biology

National projects

Lithuanian Science Programmes and Projects supported by Research Council of Lithuania 

Projects Supported by private funding “Future Biomedicine foundation”

•  “CAR-NK cell molecular research in in vitro and in vivo”, 2023-2025, Dr V. Borutinskaitė.

National projects

Lithuanian Science Programmes and Projects supported by Research Council of Lithuania 

Targeted Research in Smart Specialization Areas 

• Designing of the patient-specific, heterogeneous lung cancer cell ex vivo model system for drug efficiency prediction in personalized oncotherapy, dr. A. Kalvelyte, 2018 – 2022.

National research programm "Healthy ageing"

• Development of Innovative Targeted Therapies and Prognostic Tools for Chemotherapy-Resistant Acute Myeloid Leukemias, projekto vadovė prof. dr. R Navakauskienė, 2020-2021. 
• The Role of Molecular Modulators in the Hematological System During Cell Senescene, Differentation and Regeneration, prof. R. Navakauskienė, 2015-2018 m.

National research programm „Chronic non-infectiuos diseases“ 

• Multiplexed Molecular Biomarker Study for Leukemia, dr. R. Navakauskienė, 2012-2014
• Diagnostic and Prognostic Markers in Leukaemia, dr. R. Navakauskienė, 2010-2011
• Prognostic Markers of Stress Kinase JNK Action for Targeted Therapy, dr. A. V. Kalvelytė, 2010-2011.

Research groups projects

• Regulation of amniotic fluid‐derived stem cell functioning by microRNA and epigenetic factors, dr. J. Savickienė, 2015-2018
• Cytological, molecular and epigenetic studies of amniotic fluid, dr. G.Treigytė, 2013-2015

 Projects Supported by Vilnius University funding:

•  Search for markers of implantation potential in culture medium of human blastocysts, project No. MSFJM-10/2022, 2022-2023. Dr. A. Zentelytė.
•  Evaluation of the applicability of extracellular vesicles from mesenchymal stem cells for medication-resistant depression disorder treatment”, project No. MSF-LMT-3/2020, 2020-2024.  Dr. G. Valiulienė
•  The Effect of Probiotic Bacteria on Decidualization of Human Reproductive System Stromal Cells, project No. MSF-JM-19/2023, 2023-2024.  E. Valatkaitė.
•   Exploring the Physiological Traits and Dynamics of Treatment Resistant Depression: A Biomarker Analysis. dr. G. Valiulienė, 2019-2020 m. 

2023

1. Baušytė R., B. Vaigauskaitė Mažeikienė, V. Borutinskaitė, E. Valatkaitė, J. Besusparis, R.B. Valkiūnienė, E. Kazėnaitė, D. Ramašauskaitė, R. Navakauskienė. 2023. Human endometrium-derived mesenchymal stem/stromal cells application in endometrial-factor induced infertility. Frontiers in Cell and Developmental Biology 11: 1227487
2. Gramignoli R., N. Hofmann, M. Agudo-Barriuso, M. Antica, A. Flores, L. Girandon, H. Kerdjoudj, R. Navakauskienė, J. Schiavi, H. Scholz, V. Shablii, X. Lafarge, F.J. Nicolas, F. Gindraux. 2023. Expert revision of key elements for clinical-grade production and qualification of perinatal derivatives. Stem Cells Translational Medicine DOI 10.1093/stcltm/szad068
3. Hofmann N., X. Lafarge, M. Antica, N. Ferry, L. Girandon, R. Gramignoli, M. Jurga, H. Kerdjoudj, R. Navakauskienė, J. Schiavi 12, V. Shablii, F. J. Nicolás, F. Gindraux. 2023. Expert consideration on regulatory aspects for perinatal derivatives in clinical settings. Stem Cells Translational Medicine 12(5): 258-265.
4. Navakauskienė R., D. Žukauskaitė, V.V Borutinskaitė, T. Bukreieva, G. Skliutė, E. Valatkaitė, A. Zentelytė, L. Piešinienė, V. Shablii. 2023. Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells. Frontiers in Bioengineering and Biotechnology 11:1140781.
5. Skliutė G., B. Vaigauskaitė-Mažeikienė, A. Kaupinis, M. Valius, E. Kazėnaitė, R. Navakauskienė. 2023. The effects of the follicle-stimulating hormone on human follicular fluid-derived stromal cells. International Journal of Molecular Sciences 24(3): 2450.
6. Stulpinas A., M. Sereika, A. Vitkevičienė, A. Imbrasaitė, N. Krestnikova, A. Kalvelytė. 2023. Crosstalk between protein kinases AKT and ERK1/2 in human lung tumor-derived cell models. Frontiers in Oncology 12: 1045521.
7. Vaigauskaitė-Mažeikienė B., R. Baušytė, E. Valatkaitė, R. Mazelytė, E. Kazėnaitė, D. Ramasauskaitė, R. Navakauskienė. 2023. Assisted reproductive technology outcomes and gene expression in unexplained infertility patients. Frontiers in Cell and Developmental Biology. 11: 1217808.
8. Valiulienė G., A. Zentelytė, E. Beržanskytė, R. Navakauskienė. 2023.Effect of 3D spheroid culturing on NF-kappa B signaling pathway and neurogenic potential in human amniotic fluid stem. International Journal of Molecular Sciences 24(4): 3584.
9. Valiulienė G., V. Valiulis, A. Zentelytė, K. Dapšys, A. Germanavičius, R. Navakauskienė. 2023. Anti-neuroinflammatory microRNA-146a-5p as a potential biomarker for neuronavigation-guided rTMS therapy success in medication resistant depression disorder. Biomedicine&Pharmacotheraphy 166: 115313.
10. Žukauskaitė D., A. Vitkevičienė, A. Žlibinaitė, R. Baušytė, D. Ramašauskaitė, R. Navakauskienė. 2023. Histone H4 hyperacetylation but not DNA methylation regulates the expression of decidualization-associated genes during induced human endometrial stromal cells decidualization. International Journal of Biochemistry & Cell Biology 156: 106362.

2022

1. Borutinskaitė V., A.Zučenka, A. Vitkevičienė, M. Stoškus, A. Kaupinis, M. Valius, E. Gineikienė, R. Navakauskienė. 2022. Genetic and epigenetic signatures in acute promyelocytic leukemia treatment and molecular remission. Frontiers in Genetics 13: 821676. 
2. Čeksterytė V., A. Kaupinis, K. Jaskunė, G. Treigytė, R. Navakauskienė. 2022. Characteristics of honey bee physiological proteins extracted from faba bean (Vicia faba L.) honey. Journal of Apicultural Research DOI 10.1080/00218839.2022.2070099.
3. Navakauskienė R., S. Baronaitė, D. Matuzevičius, N. Krasovskaja, G. Treigytė, A. Arlauskienė, D. Navakauskas. 2022. Comparative proteomic assessment of normal vs. polyhydramnios amniotic fluid based on computational analysis. Biomedicines 10(8): 1821. 
4. Skliutė G., R. Baušytė, D. Ramašauskaitė, R. Navakauskienė. 2022. Characterization of epigenetic and molecular factors in endometrium of females with infertility. Biomedicines 10(6): 1324. 
5. Vaigauskaitė B., R. Baušytė, E. Valatkaitė, G. Skliutė, E. Kazėnaitė, D. Ramašauskaitė, R. Navakauskienė. 2022. Prognostic gene predictors of gestational diabetes in endometrium and follicular fluid of women after infertility. Medicina-Lithuania 58(4): 498.
6. Vitkevičienė A., G. Skliutė, A. Žučenka, V. Borutinskaitė, R. Navakauskienė. 2022. Potential prognostic markers for relapsed/refractory vs. responsive acute myeloid leukemia. Cancers 14(11): 2752. 

2021

1. Čeksterytė V., G. Treigytė, D. Matuzevičius, K. Jaškūnė, D. Navakauskas, B. Kurtinaitienė, R. Navakauskienė. 2021. Comparative proteomic profile in bee- and manually-collected Taraxacum officinale pollen. Journal of Apicultural Research doi 10.1080/00218839.2021.1969819  
2. Olejarczyk E., A. Jozwik, V. Valiulis, K. Dapšys, G. Gerulskis, A. Germanavičius. 2021. Statistical analysis of graph-theoretic indices to study EEG-TMS connectivity in patients with depression. Frontiers in Neuroinformatics 15: 651082.  
3. Olejarczyk E., V. Valiulis, K. Dapšys, G. Gerulskis, A. Germanavičius. 2021. Effect of repetitive transcranial magnetic stimulation on fronto-posterior and hemispheric asymmetry in depression. Biomedical Signal Processing and Control 68: 102585. 
4. Skliutė G., R. Baušytė, V.V. Borutinskaitė, G. Valiulienė, A. Kaupinis, M. Valius, D. Ramašauskaitė, R. Navakauskienė. 2021. Menstrual blood-derived endometrial stem cells' impact for the treatment perspective of female infertility. International Journal of Molecular Sciences 22(13): 6774. 
5. Stulpinas A., T. Užusienis, A. Imbrasaitė, N. Krestnikova, A. Ungurytė, A.V. Kalvelytė. 2021. Cell-cell and cell-substratum contacts in the regulation of MAPK and Akt signalling: Importance in therapy, biopharmacy and bioproduction. Cellular Signalling 84: 110034  
6. Valatkaitė E., R. Baušytė, A. Vitkevičienė, D. Ramašauskaitė, R. Navakauskienė. 2021. Decidualization potency and epigenetic changes in human endometrial origin stem cells during propagation. Frontiers in Cell and Developmental Biology 9: 765265. 
7. Valiulienė G., V. Valiulis, K. Dapšys, A. Vitkevičienė, G. Gerulskis, R. Navakauskienė, A. Germanavičius. 2021. Brain stimulation effects on serum BDNF, VEGF, and TNF alpha in treatment-resistant psychiatric disorders. European Journal of Neuroscience 53(11): 3791-3802. 
8. Valiulienė G., A. Vitkevičienė, G. Skliutė, V.V. Borutinskaitė, R. Navakauskienė. 2021. Pharmaceutical drug metformin and MCL1 inhibitor S63845 exhibit anticancer activity in myeloid leukemia cells via redox remodeling. Molecules 26(8): 2303. 
9. Valiulienė G., A. Zentelytė, E. Beržanskytė, R. Navakauskienė. 2021. Metabolic profile and neurogenic potential of human amniotic fluid stem cells from normal vs. fetus-affected gestations. Frontiers in Cell and Developmental Biology 9: 700634. 
10. Zentelytė A., D. Žukauskaitė, I. Jačerytė, V.V. Borutinskaitė, R. Navakauskienė. 20201. Small molecule treatments improve differentiation potential of human amniotic fluid stem cells. Frontiers in Bioengineering and Biotechnology 9, 623886. 

Monograph

1. Navakauskienė Rūta, Navakauskas Dalius, Borutinskaitė Veronika Viktorija, Matuzevičius Dalius. Epigenetics and proteomics of leukemia. A synergy of experimental biology and computational informatics. Springer, 2021. 406 p. ISBN 9783030687076

2020

1. Gasiūnienė M., E. Valatkaitė, A. Navakauskaitė, R. Navakauskienė. 2020. Long-term cultivation of human amniotic fluid stem cells: The impact on proliferative capacity and differentiation potential. International Journal of Molecular Sciences 21(22): 8752.
2. Gasiūnienė M., E. Valatkaitė, R. Navakauskienė. 2020. Long-term cultivation of human amniotic fluid stem cells: The impact on proliferative capacity and differentiation potential. Journal of Cellular Biochemistry 21: 3491-3501. 
3. Silini A.R., R. Di Pietro, I. Lang-Olip, F. Alviano, A. Banerjee, M. Basile, V. Borutinskaitė,G. Eissner, A. Gellhaus, B. Giebel, Yong-Can Huang, A. Janev, M. E. Kreft, N Kupper, A. C. Abadía-Molina, E. G Olivares, A. Pandolfi, A. Papait, M. Pozzobon, C. Ruiz-Rui, O. Soritau, S. Susman, D. Szukiewicz, A. Weidinger, S. Wolbank, B. Huppertz, O. Parolini. 2020. Perinatal derivatives: where do we stand? A roadmap of the human placenta and consensus for tissue and cell nomenclature. Frontiers in Bioengineering and Biotechnology 8: AN 610544.
4. Valiulienė G., A. Vitkevičienė, R. Navakauskienė. 2020. The epigenetic treatment remodel genome-wide histone H4 hyper-acetylation patterns and affect signaling pathways in acute promyelocytic leukemia cells. European Journal of Pharmacology 889: 173641 
5. Zentelytė A., M. Gasiūnienė, G. Treigytė, S. Baronaitė, J. Savickienė, V. Borutinskaitė, R. Navakauskienė. 2019. Epigenetic regulation of amniotic fluid mesenchymal stem cell differentiation to the mesodermal lineages at normal and fetus-diseased gestation. Journal of Cellular Biochemistry 121: 1811-1822. 

2019

1. Borutinskaitė V., G. Treigytė, D. Matuzevičius, V. Čeksterytė, B. Kurtinaitienė, A. Serackis, D. Navakauskas, R. Navakauskienė. 2019. Proteomic studies of honeybee- and manually-collected pollen. Zemdirbyste-Agriculture 106: 183-190. 
2. Čeksterytė V., Borutinskaitė V., D. Matuzevičius, G. Treigytė, D. Navakauskas, B. Kurtinaitienė, R. Navakauskienė. 2019. Evaluation of proteome profiles of Salix spp. pollen and relationship between glucose oxidase activity and pollen content in willow honey. Baltic Forestry 25: 83-96. 
3. Gasiūnienė M., G. Petkus, D. Matuzevičius, D. Navakauskas, R. Navakauskienė. 2019. Angiotensin II and TGF-β1 induce alterations in human amniotic fluid-derived mesenchymal stem cells leading to cardiomyogenic differentiation initiation. International Journal of Stem Cells. 12: 251-264. 
4. Gasiūnienė M., A. Zentelytė, G. Treigytė, S. Baronaitė, J. Savickienė, A. Utkus, R. Navakauskienė. 2019. Epigenetic alterations in amniotic fluid mesenchymal stem cells derived from normal and fetus-affected gestations: a focus on myogenic and neural differentiations. Cell Biology International 43: 299-312. 
5. Gasiūnienė M., A. Zentelytė, B. Wojtas, S. Baronaitė, N. Krasovskaja, J. Savickienė, B. Gielniewski, B. Kaminska, A. Utkus, R. Navakauskienė. 2019. DNA methyltransferases inhibitors effectively induce gene expression changes suggestive of cardiomyogenic differentiation of human amniotic fluid-derived mesenchymal stem cells via chromatin remodeling. Journal of Tissue Engineering and Regenerative Medicine 13: 469-481
6. Gasiūnienė M., A. Zubova, A. Utkus, R. Navakauskienė. 2019. Epigenetic and metabolic alterations in human amniotic fluid stem cells induced to cardiomyogenic differentiation by DNA methyltransferases and p53 inhibitors. Journal of Cellular Biochemistry 120: 8129-8143.
7. Vitkevičienė A., V. Janulis, A. Žučenka, V. Borutinskaitė, A. Kaupinis, M. Valius, L. Griškevičius, R. Navakauskienė. 2019. Oxidative phosphorylation inhibition induces anticancerous changes in therapy-resistant-acute myeloid leukemia patient cells. Molecular Carcinogenesis 58: 2008-2016.
8. Vitkevičienė A., G. Skiauterytė, A. Žučenka, M. Stoškus, E. Gineikienė, V. Borutinskaitė, L. Griškevičius, R. Navakauskienė. 2019. HDAC and HMT inhibitors in combination with conventional therapy: a novel treatment option for acute promyelocytic leukemia. Journal of Oncology. Article Number: 6179573. 

2018

Borutinskaitė, V., Virkšaitė, A., Gudelytė, G. and Navakauskienė, R., 2018. Green tea polyphenol EGCG causes anti-cancerous epigenetic modulations in acute promyelocytic leukemia cells. Leukemia & lymphoma, 59(2), pp.469–478.

Vitkevičienė, A., Baksienė, S., Borutinskaitė, V. and Navakauskienė, R., 2018. Epigallocatechin-3-gallate and BIX-01294 have different impact on epigenetics and senescence modulation in acute and chronic myeloid leukemia cells. European journal of pharmacology, 838, pp.32-40.

Borutinskaite, V., TREIGYTE, G., ČEKSTERYTE, V., Kurtinaitiene, B. and Navakauskiene, R., 2018. Proteomic identification and enzymatic activity of buckwheat (Fagopyrum esculentum) honey based on different assays. Journal of Food & Nutrition Research, 57(1).