Biochemical methods of analysis

Course unit title

Scientific direction

Scientific code

Faculty

Department (s)

Biochemical methods of analysis

Biochemistry N 004

Life Sciences Center

Institute of Biochemisry

Total number of credits

Mode of studies

Number of credits

Mode of studies

Number of credits

Lectures

0

Consultations

1

Self-studies

4

Seminars

1

Aims of course

To provide doctoral students with knowledge of modern methods applied in biochemical research. Doctoral students get acquainted with the biochemical basis of biomolecule detection, intermolecular interactions and the most important physical methods used in biochemical research: optical spectroscopy, surface plasmon resonance, electrochemistry, atomic force spectroscopy, etc. Particular attention is paid to the mechanisms of biomolecule recognition: enzymatic and immunological methods of analysis, artificial recognition systems - molecular imprint polymers, aptamers, phages and their combinations, biosensors and their application to bioanalysis. The following books are used in the studies, as well as collections of articles describing the applications of specific methods and their limits.

Main topics

• Biochemical basis of biomolecule detection, intermolecular interactions and most important physical methods.
• Applied methods for biochemical research: optical spectroscopy, surface plasmon resonance, electrochemistry, atomic force spectroscopy, etc.
• Mechanisms of biomolecule recognition: enzymatic and immunological methods of analysis, artificial recognition systems.
• What is newer in the field of molecular recognition: imprint polymers, aptamers, phages and their combinations; biosensors and their application to bioanalysis?

Main literature

• Clinical Chemistry: theory, analysis, and corelation. 1989. Eds. L.A. Kaplan and A.J. Pesce. Second edition. The C.V. Mosby Company. (clinical chemistry textbooks from other publishers are also available at a later date)
• Methods of Enzymatic Analysis Vol. 1. Fundamentals 1983 Ed. H.U. Bergmayer, third edition. Verlag Chemie.
• Pingoud, C. Urbanke, J. Hogget, A. Jeltsch. 2003. Biochemical Methods. Wiley-VCH
• R. Mikkelsen, E. Corton. 2004. Bioanalytical Chemistry. Wiley-Interscience
• Fundamentals and Applications. 1987. Ed. By A.P.F. Turner, I. Karube, G.S. Wilson. Oxford University Press. (Subsequent reviews of biosensors are also valid).
• J-M. Saveant. Elements of Molecular and Biomolecular Electrochemistry. 2006. John Wiley & Sons, Inc.
• Laurinavičius. 2012. Biocheminiai analizės metodai. VU leidykla.
• A collection of review articles from the world's most important scientific periodicals, updated annually.

Assessment strategy

Assessment criteria

Seminar

During the seminar, the duration of which is not less than an academic hour, the presentation and answers to the questions are evaluated. The presentation should be comprehensive, based on the latest literature when it comes to relevance, presented in a consistent and comprehensible manner.

Julija Razumienė

Approved by the Council of Doctoral School of Life Sciences Center No (4.10)600000-KT-… on the 14th of October 2021

Chairman dr. Daiva Baltriukienė