Cell technologies

Course unit title

Scientific direction

Scientific code

Faculty

Department (s)

Cell technologies

Biochemistry N 004

Life Sciences Center

Institute of Biochemisry

Total number of credits

Mode of studies

Number of credits

Mode of studies

Number of credits

Lectures

0

Consultations

0

Self-studies

8

Seminars

0

Aims of course

The aim is to provide knowledge about the structure of the membrane and its properties, existing intracellular forces. Lipid membrane models. Students will get acquainted with the active and passive transfer of materials through membranes, also get acquainted with the structure of the ion channels and their molecular mechanism of action, and will know about the protein channels that are involved in the development of the membrane potential on the nerves and other cells. The mechanism of lipid membrane interactions with proteins will be presented, which determines a signal transmission, functioning of pathogens and cellular protection responses. Students will gain knowledge about the transfer of various substances in the cell through the membrane vesicles, the molecular mechanisms of vesicles fusion with the plasma membrane. The latest technology and research methods in this topic will be presented.

Main topics

• Isolation, purification, characterization and imaging of mammalian cells.
• Primary and continous cell lines.
• Cell transformation and immortalization.
• Stem cell sources, markers and properties.
• Embryonic stem cells and their application.
• Methods for obtaining of induced pluripotent cells (iPSCs).
• Cell adhesion, migration / invasion, proliferation and differentiation.
• Cell application for therapy, tissue engineering, vaccine production and drug selection.
• 3D cell cultures.
• Organoids.
• Cell aging.
• Cell death.
• Cell encapsulation.
• Artificial cells.

Main literature

• Gary S. Stein, Maria Borowski, Mai X. Luong, Meng-Jiao Shi, Kelly P. Smith, Priscilla Vazquez, and Priscilla Vazquez. Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual. Wiley-Blackwell 2011
• R. Ian Freshney. Culture of animal cells: A manual of basic technique and specialized applications. John Wiley & Sons. Inc. 2016
• Robert Lanza, Robert Langer, and Joseph P. Vacanti. Principles of Tissue Engineering. Elsevier Inc. 2014
• Robert Lanza and Anthony Atala. Essentials of stem cell biology. 3rd edition. Elsevier Inc. 2014
• Stefan Przyborski. Technology Platforms for 3D Cell Culture: A User`s Guide. John Wiley & Sons. Inc. 2017
• Ranjna C. Dutta and Aroop K. Dutta. 3D Cell Culture : Fundamentals and Applications in Tissue Engineering and Regenerative Medicine. Pan Stanford Publishing Pte. Ltd. 2018
• Paskutinių penkerių metų straipsniai žurnaluose Cell, Molecular cell, Tissue engineering, Parts A, B, & C

Assessment strategy

Assessment criteria

Seminar

45-60 min. long oral presentation. Assessment: the quality of oral presentation and answers to the questions, the depth of knowledge, creativity, the ability to express the thought clearly. 

Daiva Baltriukienė

Approved by the Council of Doctoral School of Life Sciences Center No (4.10)600000-KT-… on the 14th of October 2021

Chairman dr. Daiva Baltriukienė