CRISPR-Cas-based genome editing tools are poised to revolutionize precision medicine. However, several challenges must be addressed before their clinical application, including the need for precise and efficient editing while minimizing off-target effects. Current strategies to tackle these challenges rely on rational or random mutagenesis of existing CRISPR-Cas editors, but both approaches are hindered by their low throughput. In this PhD project, we aim to transform the current landscape by utilizing cutting-edge laboratory evolution techniques to generate enhanced genome editors in vivo (i.e. within bacterial, yeast, or mammalian cells). By applying continuous in vivo gene mutagenesis combined with highly selective screening, we will generate a diverse array of mutants, enabling us to explore the vast potential of gene and protein mutagenesis. The ultimate goal of this project is to improve the specificity and efficiency of genome editors, overcoming existing barriers and facilitating their seamless transition into clinical applications.
Supervisor: Dr. Constantinos Patinios
Contacts:
Phone: +370 690 40 102
Programme: Biotechnology