14-3-3 are multifaceted proteins that interact with a wide range of partners and are abundant in different tissues. 14-3-3 recognises phosphorylated amino acids (serine or threonine) of targeted proteins and helps control their localisation, activity and phosphorylation. They have an extensive interaction network through which they are involved in both cancer and neurodegenerative diseases. Despite numerous research about the 14-3-3 family and their potential involvement in various disorders, knowledge about their function in protein aggregation and liquid phase-phase separation (LLPS) is still elusive.
This PhD proposal aims to investigate 14-3-3 family amyloid aggregation, phase separation and function activity through the integrative study approach. During the studies, the student will investigate 14-3-3 protein structural changes, biochemical activity and how they interact with diseases-related proteins. The methodology would include in vitro biophysical and biochemical experiments complemented with cell models to decipher and characterise 14-3-3 family members. The final expected result is a systematic study of 14-3-3 aggregation and LLPS that would contribute to resolving their involvement in proteinopathies.
Mokslinis vadovas / Supervisor: Darius Šulskis
Kontaktai / Contacts:
tel. / phone: +37069221885
Programme: Biochemistry N 004