Nucleases, enzymes that cleave nucleic acids, are traditionally used in molecular biology for DNA manipulation, including plasmid construction and sequence modification. Recently, they have emerged as potential biomarkers for various diseases, particularly cancers. Numerous studies report abnormal nuclease activity in cancer, with overexpression often observed in cancer cells. However, specific nuclease detection remains inherently challenging. While current methods, such as DNaseAlert™, exist for R&D typically quality control, they are not widely adapted for diagnostics. Fluorescence-based DNA probes with some selectivity have been developed, but their limited availability restricts broader application.
In collaboration with Taiwan’s China Medical University, the Department of Bioanalysis aims to develop biosensors for nuclease activity detection, focusing on MRE11, a nuclease linked to breast cancer progression. The aim of PhD studies – to develop electrochemical or colorimetric biosensors for nuclease activity detection. Objectives are: 1) Designing DNA substrates for selective and non-selective nuclease detection based on sequence/structural affinities; 2) Creating detection systems for nuclease activity using probe cleavage events or cleavage products; 3) Testing and optimizing these systems for complex sample analyses.
PhD studies will contribute significantly to bioelectrochemistry, surface science, and analytical chemistry, with the goal of publishing in top-tier journals such as ACS Sensors, Biosensors&Bioelectronics and Nature Communications.
Mokslinis vadovas / Supervisor:
Dr. Dalius Ratautas
Kontaktai / Contacts:
tel. / phone: +370 607 86164
Programme: Biochemistry N 004.