Name and Surname of the Supervisor: Lina Krikščikaitė
Institution (Company), Department: VU LSC-EMBL PI
Preliminary Topic: Revealing Targeting Specificity Across Type V CRISPR Nucleases
Short Description of the Internship Work: Learning to understand the design of DNA target sequences;
Learning to prepare DNA target sequences: PCR amplification, purification;
Learning to prepare RNPs (ribonucleoproteins) by hybridizing RNA with the Cas nuclease;
Learning to cleave DNA targets with prepared RNPs;
Learning to prepare cleavage products for sequencing;
Learning to analyze cleavage products from the electrophoresis gels and NGS (Next Generation Sequencing) data
Learning basic laboratory skills like transforming bacteria with plasmids and extracting miniprep DNA; preparing electrophoresis reagents and materials; preparing media and plates for cultivating bacteria.
Qualification Requirements (Degree): BSc
Application Deadline: 2025-06-10
Additional Comments: Requirements:
spending 5-10 hours per week at the laboratory;
consistency and wilingness to learn new skills;
attending the lab meeting (happens once a week);
checking in with me about recent progress (once a week);
fluent English language skills