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Edita Kriukiene  

Phone: +370 5 2234351 
Email:

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Research Interests: Targeted DNA labelling, DNA modifying enzymes, enzyme engeneering, nontypical methyltransferase reactions, synthetic SAM cofactors
Epigenomics of higher and lower eukaryotes, DNA and RNA modifications, methods for DNA modification profiling in genomic DNA, next generation sequencing
Single-cell epigenomics, DNA modification and RNA profiling in individual cells
Epigenetic marker discovery
Epigenetic analysis of cell-free DNA for disease diagnostics
Developmental and cancer epigenomics 
Present Position: Research Professor 
Education:
 PhD in Biochemistry 
Experience (Past Positions): 2022–present - Research Professor, Department of Biological DNA Modification, Institute of Biotechnology, Life Sciences Center, Vilnius University
2015–present - Group Leader, Epigenomics group, Department of Biological DNA Modification, Institute of Biotechnology, Life Sciences Center, Vilnius University
2012–2022 - Senior Researcher, Department of Biological DNA Modification, Institute of Biotechnology, Vilnius University
2011–05 - Visiting scientist, Krembil Family Epigenetics laboratory, Centre for Addiction and Mental Health (CAMH), University of Toronto, Toronto, Canada
2008–2011 - Postdoctoral Researcher at Department of Biological DNA Modification, Institute of Biotechnology, Vilnius, Lithuania
1998–2007 - PhD student/Research Fellow (including 1.5 year maternity leave), Laboratory of Prokaryotic Gene Engineering, Institute of Biotechnology, Vilnius, Lithuania
Preparation and teaching of a short course “Epigenomics” within the Systems Biology course 2021 for MSc students in Biochemistry, Molecular Biology and Biophysics (main lecturer Prof. Saulius Serva, VU, Life Sciences Center). 
Supervision of Doctoral Students: Janina Ličytė (2013–2018), biochemistry, Vilnius University, PhD project: “New approaches for profiling of epigenetic DNA modifications”.
Milda Narmontė (2016–2021), biochemistry, Vilnius University, PhD project: “Single-cell epigenomics. DNA modification analysis in NB”.
Kotryna Skardžiūtė (2020–present), biochemistry, Vilnius University, PhD project: “DNA modification and chromatin dynamics in development”. 
Awards: 2013 Rector’s Outstanding Scientist Award, Vilnius University.
2015 Prize for the best project in Applied Sciences (3 authors), Vilnius University.
2020 Rector’s Outstanding Scientist Award, Vilnius University. 
Publications:

LABT_LMAVB

1. Ličytė J., Kvederavičiūtė K., Rukšėnaitė A., Godliauskaitė E., Gibas P., Tomkutė V., Petraitytė G., Masevičius V., Klimašauskas S., Kriukienė E. (2022) Distribution and regulatory roles of oxidized 5-methylcytosines in DNA and RNA of the Basidiomycete fungi Laccaria bicolor and Coprinopsis cinereal. Open Biol. 12: 210302. https://doi.org/10.1098/rsob.210302. IF 5.93. Q1.
2. Narmonte M., Gibas P. Daniūnaitė K., Gordevičius J., Kriukiene E. (2021). Multiomics Analysis of Neuroblastoma Cells Reveals a Diversity of Malignant Transformations. Front Cell Dev Biol 9, 727353. IF 6.68. Q1.doi: 10.3389/fcell.2021.727353.
3. Gordevičius J, Narmontė M, Gibas P, Kvederavičiūtė K, Tomkutė V, Paluoja P, Krjutškov K, Salumets A, Kriukienė E. (2020) Identification of fetal unmodified and 5-hydroxymethylated CGs in maternal cell-free DNA for non-invasive prenatal testing, Clin Epigenetics, 12, 153. IF 6.03. Q1. doi: 10.1186/s13148-020-00938-x.
4. Ličytė J, Gibas P, Skardžiūtė K, Stankevičius V, Rukšėnaitė A, Kriukienė E. (2020) A bisulfite-free approach for base-resolution analysis of genomic 5-carboxylcytosine. Cell Reports, IF 9.4. 32:108155. doi: 10.1016/j.celrep.2020.108155.
5. Gibas P, Narmontė M, Staševskij Z, Gordevičius J, Klimašauskas S, Kriukienė E. (2020) Precise genomic mapping of 5-hydroxymethylcytosine via covalent tether-directed sequencing. PLoS Biol. 18(4):e3000684. IF 8.3. doi: 10.1371/journal.pbio.3000684.
6. Daniūnaitė K, Jarmalaitė S, Kriukienė E. (2019) Epigenomic technologies for deciphering circulating tumor DNA. Curr Opin Biotechnol. 55:23-29. IF 8.3. doi: 10.1016/j.copbio.2018.07.002.
7. Staševskij Z., Gibas P., Gordevičius J., Kriukienė E.* and Klimašauskas S.* (2017) Tethered Oligonucleotide-Primed sequencing, TOP-seq: a high-resolution economical approach for DNA epigenome profiling. Mol Cell, 65, 1-11. * - corresponding authors. IF 14.708. doi: 10.1016/j.molcel.2016.12.012.
8. Labrie V., Buske O. J, Oh E., Jeremian R., Ptak C., Gasiūnas G., Maleckas A., Petereit R., Žvirbliene A., Adamonis K., Kriukienė E., Koncevičius K., Gordevičius J., Nair A., Zhang A., Ebrahimi S., Oh G., Šikšnys V., Kupčinskas L., Brudno M., and Petronis A. (2016) Lactase nonpersistence is directed by DNA-variation-dependent epigenetic aging. Nature Struct. Mol. Biol. 23: 566-573. IF 13.338. doi: 10.1038/nsmb.3227.
9. Tomkuvienė M, Kriukienė E, Klimašauskas S. (2016) DNA Labeling Using DNA Methyltransferases. Adv Exp Med Biol. 945, 511-535. IF 2.5. doi: 10.1007/978-3-319-43624-1_19.
10. Liutkevičiūtė Z. & Kriukienė E., Ličytė J, Rudytė M, Urbanavičiūtė G, Klimašauskas S. (2014) Direct decarboxylation of 5-carboxylcytosine by DNA C5-methyltransferases. J Am Chem Soc. 136, 5884-7. IF 12.113. doi: 10.1021/ja5019223.
11. Kriukiene E., Labrie V., Khare T., Urbanavičiūtė G., Lapinaitė A., Koncevičius K., Li D., Wang T., Pai S., Ptak C., Gordevičius J., Wang SC., Petronis A., Klimašauskas S. (2013) DNA unmethylome profiling by covalent capture of CpG sites. Nat Commun, 4:2190. IF 10.742. doi: 10.1038/ncomms3190.
12. Khare T., Pai S., Koncevicius K., Pal M., Kriukienė E., Liutkevičiūtė Z., Irimia M., Jia P., Ptak C., Xia M., Tice R., Tochigi M., Moréra S., Nazarians A., Belsham D., Wong A.H., Blencowe B.J., Wang S.C., Kapranov P., Kustra R., Labrie V., Klimasauškas S., Petronis A. (2012) 5-hmC in the brain is abundant in synaptic genes and shows differences at the exon-intron boundary. Nat Struct Mol Biol. 19, 1037-43. IF 11.902. doi: 10.1038/nsmb.2372.
13. Kriukienė E., Liutkevičiūtė Z., Klimašauskas S. (2012) 5-Hydroxymethylcytosine - the elusive epigenetic mark in mammalian DNA. Chem Soc Rev., 41, 6916-30. IF 24.892. doi: 10.1039/c2cs35104h.
14. Liutkevičiūtė Z., Kriukienė E., Grigaitytė I., Masevičius V., Klimašauskas S. Methyltransferase-directed derivatization of 5-hydroxymethylcytosine in DNA. (2011) Angew Chem Int Edit. 50, 2090-3. Angew Chem. (2011), 123, 2138-2141. IF 9.24. doi: 10.1002/anie.201007169.
15. Jakubauskas A. Kriukienė E. Trinkūnaitė L., Šapranauskas S. Jurėnaitė-Urbanavičienė S., Lubys A. Bioinformatic and partial functional analysis of pEspA and pEspB, two plasmids from Exiguobacterium arabatum sp. nov. RFL1109. (2009) Plasmid. 61, 52-64. doi: 10.1016/j.plasmid.2008.09.004.
16. Klimašauskas S., Gerasimaitė R., Urbanavičiūtė G., Kriukienė E., Lukinavičius G., Petronis A., Epigenome profiling via DNA methyltransferase-directed labeling (2008) Cellular Oncology, 30, 232.
17. Jurėnaitė-Urbanavičienė S., Šerkšnaitė J., Kriukienė E., Giedrienė J., Venclovas C., Lubys A.. Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains. (2007) Proc. Natl. Acad. Sci. USA 104, 10358-10363. IF 9.6. doi: 10.1073/pnas.0610365104.
18. Kriukienė E. Domain organization and metal ion requirement of the type IIS restriction endonuclease MnlI. (2006) FEBS Letters 580, 6115-6122. IF 3.7. doi: 10.1016/j.febslet.2006.09.075.
19. Kriukienė E., Lubienė J., Lagunavicius A., Lubys A. MnlI-The member of H-N-H subtype of Type IIS restriction endonucleases. (2005) Biochim. Biophys. Acta 1751, 194-204. IF 2.5. doi: 10.1016/j.bbapap.2005.06.006.
Academic Cooperation: Prof. Arturas Petronis. Analysis of DNA modification in diseases. CAMH, University of Toronto, Canada. 
Prof. Andres Salumets. Collaboration on cell-free DNA analysis. Tartu University, Competence centre on Health Technologies, Estonia. 
Prof. Haruhiko Koseki. Analysis of CGI Polycomb complexes. RIKEN Yokohama, Japan. 
Prof. K Gurova. Research of cancer cells, cell resistance to drugs. Department of Cell Stress Biology, Roswell Park Comprehensive Cancer Center, Buffalo, USA. 

Projects and Grants:

Project involvement (over the past 5 years):
2021–2024 - S-MIP-21-1. DNA modification and chromatin dynamics during growth and differentiation of healthy and malignant cells. Research Council of Lithuania. Role: PI.
September 2016–February 2023 - European research Council (ERC-AdG-2016, 742654) "Single-cell temporal tracking of epigenetic DNA marks" (EpiTrack). Role: senior researcher.
2018–2022 - 09.3.3-LMT-K-712-01-0041 European Social Fund, the activity measure No. 09.3.3-LMT-K-712 “Improvement of researchers’ qualification by implementing world-class R&D projects” under grant agreement with the Research Council of Lithuania. “Single molecule TOP-seq – an innovative technological platform for early non-invasive diagnostics of cancer and other epigenetic disorders”. Role: PI.
2017–2020/11 MIP-17-58. “A technology for single-cell analysis of genomic DNA modification. Neuroblastoma epigenetic heterogeneity”. Research Council of Lithuania. Role: PI.
2013–2016 - MIP-045/2013. “Genome wide analysis of CpG sites”, Research Council of Lithuania. Role: PI.
Qualification Improvement, Certificates: 2008 09 TATAA Biocenter – Training course in real-time PCR, Freising, Germany
2014 Ion Torrent Proton System. Hands-on Training and Data Analysis by Life Technologies; Vilnius, Lithuania 
Academic Dissemination: 2015, 2016 A science education lecture for secondary school children “What is Epigenomics”.
February 28, 2017 DELFI: Lithuanian biotechnologists were included in a world-famous publication
June 2017, journal Spectrum; 1(26)/2017. E. Kriukienė. Unikalūs molekuliniai įrankiai padės pastebėti artėjančią ligą.
September 07, 2017 Lietuvos žinios. E. Kriukienė. Unikalūs molekuliniai įrankiai padės pastebėti artėjančią ligą.
September 23, 2017 15 min. VU researchers have discovered a unique method for detecting impending diseases
Languages: Lithuanian (native), English, German, Russian

 

Research

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