| Publications: |
LABT_LMAVB
1. Tautvydas Karvelis , Gytis Druteika, Greta Bigelyte, Karolina Budre , Rimante Zedaveinyte , Arunas Silanskas , Darius Kazlauskas , Česlovas Venclovas , Virginijus Siksnys (2021) Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease. Nature. 2021; 599(7886): 692–696.
2. Golovinas E, Rutkauskas D, Manakova E, Jankunec M, Silanskas A, Sasnauskas G, Zaremba M. (2021) Prokaryotic Argonaute from Archaeoglobus fulgidus interacts with DNA as a homodimer. Sci Rep. 2021; 11: 4518. Published online 2021 Feb 25. doi: 10.1038/s41598-021-83889-4.
3. Karvelis T, Bigelyte G, Young J, Hou Z, Zedaveinyte R, Budre K, Paulraj S, Djukanovic V, Gasior S, Silanskas A, Venclovas Č, Siksnys V. (2020) PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage. Nucleic Acids Res. 2020 May 21; 48(9): 5016–5023. Published online 2020 Apr 4. doi: 10.1093/nar/gkaa208.
4. Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB. (2019) Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System. Mol. Cell. 2019 Jul 11;75(1):90-101.e5. doi: 10.1016/j.molcel.2019.04.020. Epub 2019 May 9.
5. Drabavicius G, Sinkunas T, Silanskas A, Gasiunas G, Venclovas Č, Siksnys V. (2018) DnaQ exonuclease-like domain of Cas2 promotes spacer integration in a type I-E CRISPR-Cas system. EMBO Rep. 2018 Jul;19(7). pii: e45543. doi: 10.15252/embr.201745543. Epub 2018 Jun 11.
6. Toliusis P, Tamulaitiene G, Grigaitis R, Tuminauskaite D, Silanskas A, Manakova E, Venclovas C, Szczelkun MD, Siksnys V, Zaremba M. (2018) The H-subunit of the restriction endonuclease CglI contains a prototype DEAD-Z1 helicase-like motor. Nucleic Acids Res. 2018 Mar 16;46(5):2560-2572. doi: 10.1093/nar/gky107.
7. Toliusis P, Zaremba M, Silanskas A, Szczelkun MD, Siksnys V. (2017) CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases. Nucleic Acids Res. 2017 Aug 21;45(14):8435-8447. doi: 10.1093/nar/gkx580.
8. Karvelis T, Gasiunas G, Young J, Bigelyte G, Silanskas A, Cigan M, Siksnys V (2015) Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements. Genome Biol. 16:253. doi: 10.1186/s13059-015-0818-7.
9. Zaremba M, Toliusis P, Grigaitis G, Manakova M., Silanskas A, Tamulaitiene G, Szczelkun MD & Siksnys V (2014) DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis. Nucleic Acids Research. 42(22):13887-96. doi: 10.1093/nar/gku1236.
10. Tamulaitiene G, Silanskas A, Grazulis S, Zaremba M & Siksnys V (2014) Crystal structure of the R-protein of the multisubunit ATP-dependent restriction endonuclease NgoAVII. Nucleic Acids Research, 42(22):14022-30. doi: 10.1093/nar/gku1237.
11. Silanskas A, Zaremba M, Sasnauskas G, Siksnys V (2012) Catalytic activity control of restriction endonuclease--triplex forming oligonucleotide conjugates. Bioconjug Chem. 23(2):203-11. doi: 10.1021/bc200480m.
12. Silanskas A, Foss M, Wende W, Urbanke C, Lagunavicius A, Pingoud A, Siksnys V. (2011) Photocaged variants of the MunI and PvuII restriction enzymes. Biochemistry. 2011 Apr 12;50(14):2800-7. doi: 10.1021/bi2000609. Epub 2011 Mar 16.
13. Geel TM, Meiss G, van der Gun BT, Kroesen BJ, de Leij LF, Zaremba M, Silanskas A, Kokkinidis M, Pingoud A, Ruiters MH, McLaughlin PM, Rots MG. (2009) Endonucleases induced TRAIL-insensitive apoptosis in ovarian carcinoma cells. Exp Cell Res. 2009 Sep 10;315(15):2487-95. doi: 10.1016/j.yexcr.2009.06.011. Epub 2009 Jun 18.
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